Significance of Detection of Serum Cystatin C,Macrophage Migration Inhibitory Factor and Urinary Podocytes in Children with Primary Nephrotic Syndrome / 实用儿科临床杂志

ABSTRACT

Objective To detect the level of serum cystatin C(CysC),macrophage migration inhibitory factor(MIF),urinary podocytes(UPC) and explore the correlation between the levels of the three and therapeutic effect in children with primary nephrotic syndrome(PNS).Methods The serum CysC,MIF levels were detected in 38 children with PNS,which were treated before and after remission by double-antibody sandwich sensitive enzyme-linked immunosorbent assay(ELISA).Podocyte-specific marker protein-Podocalyxin(PCX) was detected by immunofluorescene to definite UPC before and after remission and compare with those of healthy control group(n=15).Results Compared with control group,the levels of serum MIF were significantly increased in 22 patients responded sensitively to prednisone prior and post treatment(Pa0.05),however,CysC,MIF and UPC significantly increased after treatment in 16 steroid-resistant children(Pa0.05), MIF significantly decreased(Pa0.05)in steroid-resistant children after treatment.In 16 children with steroid-resistant, complete remission of PNS as cyclophosphamide pulse treatment being added in 10 patients,which CysC, MIF,UPC were significantly higher than those in control group(Pa