Changes of Intestinal Flora of Children with Pneumonia Detected by Fluorescent Quantitative 16S rRNA/DNA Targeted Polymerase Chain Reaction Technique / 实用儿科临床杂志

ABSTRACT

Objective To study the intestinal flora changes of children with pneumonia,and explore the feasibility and practicability of fluorescent quantitative 16S rRNA/DNA targeted polymerase chain reaction(PCR) in quantitative study of bacterium.Methods The bacterial DNA was extracted in stool between 23 healthy children (control group)and 23 children (pneumonia group)with pneumonia after therapy,A260 of bacteria was detected and compared between 2 groups.16S rRNA/DNA PCR were applied to analyze and compare the bacterial content of lactobacilli and Escherichia coli in stool between control group and pneumonia group.Results Bacterial A260 of stool were respectively(3 381.2?817.2)mg/L in control group,(1 643.5?498.4)mg/L on therapy group post-treatment first day,(859.6?165.2) mg/ L on the third day of post-treatment,(1 263.8?337.3)mg/ L on the 7th day.There were significant differences of bacterial A260 between control group and pneumonia group in post-therapy(Pa