Inhibitory effect of lipoic acid on apoptosis of neurons during neuronal aging / 上海第二医科大学学报

ABSTRACT

Objective To study the effect of lipoic acid on apoptosis of neurons during experimental neuronal aging. Methods The mouse neuroblastoma cells with serum-free culture were served as experimental neuronal aging models.The cells were randomly divided into control group(serum-free culture)and experimental group(100?mol/L lipoic acid in serum-free culture).After cultivating for 5,10 and 15 d,the cell survival rates were detected by MTT method,and the cell apoptosis rates were quantitatively determined using FACS with Annexin V/PI double staining.Results Compared with those in control group,the cell survival rates in experimental group significantly increased[(212.48?12.26)%vs(100.00?14.80)%,(200.55?28.33)%vs(75.45?10.79)%and(96.81?14.50)%vs(28.54?3.14)%,respectively for 5, 10 and 15 d after cultivation](P