Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells / 中国病毒学
Virologica Sinica
;
(4): 181-1999.
Article
in Chinese
| WPRIM
| ID: wpr-640967
ABSTRACT
The coding region of S genome segment of Hantaan virus (76/118 strain) was inserted into the eukarytic expression plasmidpVR1012. The recombinant expression plasmid pVRS22 was constructed. Vero-E6 cells were transiently transfected in vitro with pVRS22 plasmid. The transient expression of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indirect immunofluorescence assay (IFA) with monoclonal antibody 5H5 against Hantaan virus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Virologica Sinica
Year:
1999
Type:
Article
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