[Ca2+]i homeostasis and caspase-3 gene expression in verapamil-induced retinal pigment epithelium cells apoptosis in vitro / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 1-3, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-641399
ABSTRACT
(Ver)-induced human retinal pigment epithelium (RPE) cells apoptosis.hours to induce RPE cells apoptosis.The expression of apoptotic effector gene caspase-3 was assessed by reverse transcription polymerase chain reaction (RT-PCR).Single cell was measured using fluorescence indicator Fura-3/AM with MetaFluo4.5/coolsnapfx/IX70 intracellular Ca2+ fluorescence imaging system.RPE cells and it significantly increased after co-cultured with Ver.The fluorescence in resting RPE cells was strong and distributed throughout the cells.The nucleus appeared more fluorescent than the cytoplasm.Calcium fluorescence of RPE cells attenuated after co-cultured with Ver.[Ca2+]i homeostasis might play pivotal roles in Ver-induced RPE cells apoptosis.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2011
Type:
Article
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