Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigment epithelial cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 409-412, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-641446
ABSTRACT
AIM:
To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC).METHODS:
Human fetal BMSC were previously labeled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial (RPE) cells which were pre-treated with ultraviolet irradiation at a ratio of 1∶1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC.RESULTS:
The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE.CONCLUSION:
Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2010
Type:
Article
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