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Effects of endothelin-1 on the cytoskeleton protein F-actin of human trabecular meshwork cells in vitro / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science ; (12): 209-211, 2010.
Article in Chinese | WPRIM | ID: wpr-641452
ABSTRACT

AIM:

To observe the effect of endothelin-1(ET-1) on the cytoskeleton protein F-actin of cultured human trabecular meshwork (HTM) cells.

METHODS:

Cultured HTM cells were randomly divided into four groups control group(0mol/L), low-dose ET-1(10-9mol/L) treatment group, middle-dose ET-1(10-8 mol/L) treatment group, and high-dose ET-1(10-7 mol/L) treatment group. After treated with ET-1, the expression of cytoskeleton protein F-actin in trabecular meshwork was analyzed with Western-blot and the distribution of F-actin was detected with FITC-Phalloidin probe.

RESULTS:

ET-1 dose-dependently and significantly increased F-actin in trabecular meshwork cells. The F-actin stress fiber and periphery actin fiber highly increased and manifested mild reorganization after treated with ET-1; and there were much more cell-to-cell and cell-to-extracellular matrix attachments formation in ET-1 treated HTM cells than that in the untreated HTM cells.

CONCLUSION:

ET-1 promoted the expression of cytoskeleton protein F-actin and induced the trabecular meshwork actin cytoskeleton reorganization.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Eye Science Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Eye Science Year: 2010 Type: Article