Preliminary findings of immunological characteristics in primary human corneal endothelial cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 1221-1223, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-641513
ABSTRACT
·AIM:
To evaluate the function of primary human corneal endothelial cells(HCEC) serving as immunological cells. ·METHODS:
Expression of HLA-DP, -DQ, -DR, CD40, CD80, and CD86 was determined by immunohistochemical methods. Meanwhile, purified peripheral blood mononuclear cells(PBMC) were cocultured with primary HCEC which were pre-treated with and without γ-IFN respectively; activation of lymphocytes was determined by FACS analysis.·RESULTS:
In coculture system, T lymphocyte was activated by primary HCEC, HLA-DP, -DQ, -DR and CD40 expression were increased by γ-IFN induction. Costimulatory molecular CD80 was shown on the endothelial cells. ·CONCLUSION:
Primary HCEC were assumed to be involved in the corneal transplantation rejection process as potential antigen presenting cells(APC).
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2009
Type:
Article
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