A feasibility study of recombinant adeno-associated virus(rAAV)as a vector for transferring a target gene to retina / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 1740-1742, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-641561
ABSTRACT
AIM:
To study the feasibility of recombinant adeno-associated virus(rAAV)as a vector to transfer the green fluorescent protein(GFP)gene as a target gene into rabbit retina.METHODS:
Intravitreal injection of rAAV-gfp was performed in either eye for each rabbit with the other eye taken as control.At the 3rd,7th,and 14th day after injection,the eyeballs were removed,and the retinas were flat-mounted on glass slides to inspect the retinal fluorescence,respectively.RESULTS:
After intravitreal injection of rAAV-gfp,the presence of fluorescent spots in the cytoplasm of retinal cells indicated that GFP gene was efficiently transferred and expressed in the rabbit retina.CONCLUSION:
Recombinant adeno-associated virus is a reliable and simple vector for transferring target gene,e.g.,GFP gene,to the retina.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2008
Type:
Article
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