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Role of p42/p44 MAPK signal transduction pathway in expression of VEGF induced by elevated glucose concentration in cultured hRPE cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science ; (12): 1382-1385, 2014.
Article in Chinese | WPRIM | ID: wpr-641926
ABSTRACT

AIM:

To study p42/p44 mitogen - activated protein kinases ( MAPK ) signal transduction pathway effect on vascular endothelial growth factor ( VEGF ) expression induced by elevated glucose concentration in cultured human retinal pigment epithelium ( hRPE) .

METHODS:

hRPE cells were cultured and divided into four groupsnormal glucose group (NG) (5. 6mmol/L), high glucose group ( HG115mmol/L D-glucose, HG220mmol/L D - glucose, HG330mmol/L D - glucose ), PD98059 group hRPE cells were treated by an efficient and selective inhibitor PD98059 (20μmol/L) of p42/p44MAPK signal transduction pathway and solvent dimethyl sulfoxide group ( DMSO group) . The expression of VEGF and pigment epithelium derived factor ( PEDF ) mRNA was detected by RT-PCR. VEGF protein expression in cultured hRPE supernatants was detected by enzyme-linked immumosorbent assay ( ELISA) . RUSULTS VEGF mRNA and protein expression induced by elevated glucose concentration increased significantly. VEGF mRNA and protein expression were restrained in PD98059 group. Ratio of ( VEGF/β-actine)/( PEDF/β - actine ) in PD98059 group decreased significantly compare with that in high glucose group.

CONCLUSION:

p42/p44MAPK signal transduction pathway might play a part in VEGF expression induced by elevated glucose concentration in cultured hRPE cells.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Eye Science Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Eye Science Year: 2014 Type: Article