Expression of GST-pi and MDR1 genes in operative specimens of ovarian cancer / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences
;
(6): 133-136, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-642352
ABSTRACT
Objectives:
To study the expression of GST-pi and MDR1 genes in operative specimens of ovarian cancer,and to analyze the possible clinical role of GST-pi and MDR1.Methods:
Eighteen frozen specimens of ovarian carcinoma and ten specimens of normal ovarian tissues from patients were examined for the expression of GST-pi and MDR1 genes by means of RT-PCR, and quantitative analysis was performed using β-actin as internal contrast.Results:
Positive expression rate of GST-pi and MDR1 in ovarian carcinoma were 61.1% and 33.3%,respectively,and in contrast, 20% and 10% in normal ovarian tissues respectively. The level of GST-pi gene expression in ovarian carcinoma was obviously higher than that in normal ovarian tissue (P<0.05)and MDR1 gene also had high level expression in ovarian carcinoma, but had no statistical significantance. Four patients with ovarian carcinoma had GST-pi and MDR1 coexpression. Expression levels of GST-pi mRNA were lower than that of protein.Conclusions:
(1) GST-pi and MDR1 had higher level expression in ovarian carcinoma than in normal ovarian tissues. (2) GST-pi and MDR1 may have same regulating factors but different mechanisms of action. (3)Processing after transcription and/or regulation of translation level may exist in GST-pi expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Bulletin of The Academy of Military Medical Sciences
Year:
2001
Type:
Article
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