Expression of angiotensin Ⅱ in cultured human trabecular meshwork cell / 眼科研究

ABSTRACT

Objective Research showed that angiotensin converting enzyme inhibitor and angiotensinⅡ(AngⅡ)receptor antagonist has good role of lowing-intraocular pressure.This study was to explore whether cultured human trabecular meshwork cells express AngⅡin vitro.MethodsThe human trabecular meshwork cells strains were cultured in DMEM+F12 medium containing 25% fetal bovine serum in vitro and passaged at the climbing sheet was prepared.The expression of AngⅡ in human trabecular meshwork cells was examined by immunohistochemistry,and AngⅡ protein was localized by Western blot.ResultsSubcultured cells showed spindle shape.AngⅡwas positively expressed in human trabecular meshwork cells by immunochemistry,showing the yellow-brown granule in cellular membrane and cytoplasm.A absence of response for AngⅡwas found in negative control sample.The band of AngⅡ protein was found at the relative molecular weight of 64 000 by Western blot.ConclusionThe result implies that human trabecular meshwork cells have the ability of synthesizing AngⅡ.It suggests that AngⅡ participates in the regulation of intraocular tension in glaucomous eye.