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Rapid and quantitative detection of abrin using colloid gold immunochromatographic assay / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences ; (6): 546-549, 2009.
Article in Chinese | WPRIM | ID: wpr-642527
ABSTRACT

Objective:

To develop a rapid and quantitative detection method for abrin using colloid gold immunochromatographic assay.

Methods:

The rapid detection method was established with double-antibody sandwich assay. Quantification was realized by constructing a standard curve. The specificity and sensitivity of the method were tested, and its feasibility was evaluated by various abrin-added food samples. Results and

Conclusion:

This established method could accomplish qualitative and semi-quantitative detection in 15 minutes; the sensitivity was up to 30 ng/ml with a linear range from 30 ng/ml to 600 ng/ml. The recovery rate was 80%-110%, and the variation coefficient was less than 15%.The colloidal gold immunochromatographic assay is rapid, specific, sensitive,accurate and suitable for field detection.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Qualitative research Language: Chinese Journal: Bulletin of The Academy of Military Medical Sciences Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Qualitative research Language: Chinese Journal: Bulletin of The Academy of Military Medical Sciences Year: 2009 Type: Article