Construction of a Saccharopolyspora erythraea mutant producing erythromycin B as its major fermentation product / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences
;
(6): 18-23, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-642686
ABSTRACT
Objective:
To obtain a large amount of erythromycin B and to investigate the activity site in eryK.Methods:
The key sequence of the BC loop region in eryK gene was knocked out and the eryK gene with 101 bp deleted was amplified by overlapping PCR,and cloned into vector pWHM3 to construct recombinant plasmid. The Saccharopolyspora erythraea mutant AK17 was constructed through chromosomal homologous recombination technique.Results andConclusions:
The S.erythraea mutant AK17 was constructed. The results of TCL and MS analysis showed that the major fermentation product of AK17 is erythromycin B.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Bulletin of The Academy of Military Medical Sciences
Year:
2009
Type:
Article
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