Influence of nuclear factor-κB inhibitor on cataract formation after alkali burn / 眼科研究

ABSTRACT

Objective Previous researches demonstrated that pyrroledithiocarbomate (PDTC), an inhibitor of nuclear factor, results in specific inhibition on nuclear factor-κB and therefore suppress cataract formation. The aim of this study was to explore the effect of nuclear factor-κB inhibitor on cataract formation after alkali burn in rats. Methods Ocular surface alkali burn models were established in the right eyes of 40 SPF Wistar rats by putting the 7 mm filter paper with 1 mol/L NaOH in the central cornea for 60 seconds. PDTC (2 mg/mL, 0. 1 mL) was subconjunctivaly injected everyday in 20 model eyes and the equivalent amount of normal saline solution was used in the same way in other 20 model eyes. The rats were killed in the first, third, fifth and seventh day after alkali burn and lenses were obtained for the histopathological examination, and immunochemistry and polymerase chain reaction were used to detect the expression of nuclear factor-κB in lens epithelial cells. The experiment and use of animal followed the Standard of Association for Research in Vision and Ophthalmology. Results After alkali burn, lens epithelial cells namely fell off in control group but those of experimental group were complete in the first day. In the third day, the lens cortex was obviously condensed in both two groups. In the fifth and seventh day, the lens epithelial cells fell off and lens cortex was obviously briquetted. Lots of vacuole and fragments could be seen in both groups. The gray scale value of nuclear factor-κB in lens epithelial cells were significantly higher in 1 day and 3 days after alkali burn in PDTC group compared with control group(t =2. 836, P =0. 036; t =4. 932, P =0.004) . The nuclear factor-κB /p-actin values were considerably lowed in 1 day and 3 days after alkali burn in PDTC group compared with control group (t = 31. 563, P = 0. 000; t = 17. 837, P = 0. 000). No statistically significant difference were found in gray scale values of nuclear factor-κB and nuclear factor-κB/p-actin values in 5 days and 7 days after alkali burn between PDTC group and control group (P＞0. 05). Conclusion Early usage of inhibitor of nuclear factor plays a suppressive role in cataract formation after ocular surface alkali burn of rat model.