Effect of different sampling solutions on viability of bacteriophages / 军事医学科学院院刊

ABSTRACT

Objective To investigate the endurance or resistance of different bacteriophages to bubbling stress in different sampling solutions,to select the optimum sampling solution from three different ones and to select relatively stress-resistant bacteriophages from five different ones.Methods AGI-10(all glass impinger)was used as the representative for all the impingers that would bubble during operation to fulfill the bubbling experiment.Three different sampling solutions used,such as distilled water(DW),phosphatic buffer solution(PBS),and suspension medium(SM),were divided into two groups by adding olive oil(50 μl) or otherwise(0 μl).The impingers were operated 30 min at a flow rate of 7.0 L/min.The titers of bacteriophages and the volume of final sampling solutions were determined before the corrected survival probability was used to evaluate the stress resistance of several different bacteriophages.Results It was found that the survival probability of the same bacteriophage bubbling with different sampling solutions was different except for bacteriophage F2.The use of SM as the collection fluid was related to a high survival probability which remained unchanged between 50 μl and 0 μl olive oil.The corrected survival probability was 79%,77%,86%,50% and 71% for phage SM701,SM702,PhiX174,EcP1 and F2 respectively after 60 minutes of impingement at a flow rate of 7.0 L/min.Conclusion The endurance or resistance of different kinds of bacteriophages in the same sampling solution is different.SM might be an optimum sampling solution for phages.Bacteriophage SM701,SM702 and PhiX174 are more resistant to bubbling stress than EcP1 and F2.