Morphological Study of GFAP Immunoreactive Cells on Fetuses and Neonate Telencephalon of Korean Native Goat / 대한해부학회지

ABSTRACT

GFAP (Glial Fibrillary Acidic Protein) was one of the intermediate filament group and used as an astrocyte marker. The numerous studies about GFAP immunoreactive cell's distribution were investigated for fetus, neonate and aged brains. There are several reports about that GFAP immunoreactive cells were appeared at early fetus or after birth. In cases of mammalian fetus radial glia cells migrated toward pial surface at early stage and revealed GFAP immunoreactivity by the immunostain. But in cases of rodents, they migrated last gestation or after birth. This study, the GFAP immunoreactive cells' localizations and distribution in the fetuses (the 30th, 45th, 60th, 90th, 95th, 105th 120th of gestation) and neonate telencephalon of Korean native goat were investigated by immunohisto-chemistry (ABC method). The results obtained in this study were summarized as followings; 1. Multipolar astrocytes of 60 days of gestation were found cerebral cortex, in 95 days of gestation were found cerebral medulla, in 105 days of gestation were found lateral ventricle. 2. Radial glial cell presented 45 days of gestation and process of GFAP immunoreactive was to stretch out from ventricular to pia mater. And the nonpolar immunoreactive cells were transformed bipolar immunoreactive cells and they were transformed to monopolar and multipolar immunoreactive cell. 3. The number of GFAP immunoreactive cells of a field were gradually increased from 45 days of gestation till 90 days of gestation and decreased from 90 days of gestation till 105 days of gestation. But in 120 days of gestation and newborn were slightly increased. 4. Immunoreactivity of GFAP immunoreactive cells were gradually decreased from 95 days of gestation till 120 days of gestatioin. However, most pia mater areas and ventricles had high immunoreactivity and medulla part had low immunoreactivity. These results were suggested that radial glial cell of cerebral cortex and cerebral medulla were developed faster than lateral ventricle.