Electrospun Fibrous Silk Fibroin/Poly(L-Lactic Acid) Scaffold for Cartilage Tissue Engineering
Tissue Engineering and Regenerative Medicine
;
(6): 516-526, 2016.
Article
in English
| WPRIM
| ID: wpr-644858
ABSTRACT
For successful tissue engineering of articular cartilage, a scaffold with mechanical properties that match those of natural cartilage as closely as possible is needed. In the present study, we prepared a fibrous silk fibroin (SF)/poly(L-lactic acid) (PLLA) scaffold via electrospinning and investigated the morphological, mechanical, and degradation properties of the scaffolds fabricated using different electrospinning conditions, including collection distance, working voltage, and the SFPLLA mass ratio. In addition, in vitro cell-scaffold interactions were evaluated in terms of chondrocyte adhesion to the scaffolds as well as the cytotoxicity and cytocompatibility of the scaffolds. The optimum electrospinning conditions for generating a fibrous SF/PLLA scaffold with the best surface morphology (ordered alignment and suitable diameter) and tensile strength (~1.5 MPa) were a collection distance of 20 cm, a working voltage of 15 kV, and a SFPLLA mass ratio of S50P50. The degradation rate of the SF/PLLA scaffolds was found to be determined by the SFPLLA mass ratio, and it could be increased by reducing the PLLA proportion. Furthermore, chondrocytes spread well on the fibrous SF/PLLA scaffolds and secreted extracellular matrix, indicating good adhesion to the scaffold. The cytotoxicity of SF/PLLA scaffold extract to chondrocytes over 24 and 48 h in culture was low, indicating that the SF/PLLA scaffolds are biocompatible. Chondrocytes grew well on the SF/PLLA scaffold after 1, 3, 5, and 7 days of direct contact, indicating the good cytocompatibility of the scaffold. These results demonstrate that the fibrous SF/PLLA scaffold represents a promising composite material for use in cartilage tissue engineering.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tensile Strength
/
In Vitro Techniques
/
Cartilage
/
Cartilage, Articular
/
Chondrocytes
/
Tissue Engineering
/
Silk
/
Extracellular Matrix
/
Fibroins
Language:
English
Journal:
Tissue Engineering and Regenerative Medicine
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS