Effects of Dexamethasone on RANTES Expression of Nasal Fibroblast / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 930-935, 2001.
Article
in Korean
| WPRIM
| ID: wpr-645075
ABSTRACT
BACKGROUND AND OBJECTIVES:
Fibroblasts play an indirectly augmenting effector role in allergic inflammatory response by releasing different proinflammatory cytokines, including RANTES, GM-CSF, IL-8 after stimulation by other inflammatory cytokines such as IFN-gamma, TNF-alpha. The aim of this study was to investigate expression of RANTES in allergic and non-allergic nasal fibroblasts after stimulation with IFN-gamma and TNF-alpha, and to study the effect of dexamethasone on the RANTES expression of nasal fibroblast cell. MATERIALS ANDMETHODS:
Using the 3rd passage of fibroblasts taken from the inferior turbinates of allergic and non-allergic patients, we evaluated the RANTES expression of fibroblasts after the IFN-gamma, TNF-alpha stimulation in the presence or in the absence of dexamethasone by ELISA.RESULTS:
The expression of RANTES in allergic nasal fibroblasts stimulated by cytokines was stronger than in non-allergic nasal fibroblasts stimulated by cytokines. And dexamethasone suppressed the RANTES expression in allergic nasal fibroblasts stimulated by IFN-gamma. However, dexamethasone did not affect the RANTES expression in allergic fibroblasts stimulated by TNF-alpha and non-allergic fibroblasts stimulated by IFN-gamma and TNF-alpha.CONCLUSION:
This study shows different responses of the RANTES production in nasal fibroblasts to dexamethasone, perhaps reflecting heterogeneity of nasal fibroblasts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Turbinates
/
Population Characteristics
/
Dexamethasone
/
Enzyme-Linked Immunosorbent Assay
/
Cytokines
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Interleukin-8
/
Tumor Necrosis Factor-alpha
/
Chemokine CCL5
/
Fibroblasts
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS