The Change of Molecular Event of p53 by Cisplatin and 5-Fluorouracil in Hypopharyngeal Cell Line(PNUH-12) / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 984-989, 2002.
Article
in Korean
| WPRIM
| ID: wpr-645379
ABSTRACT
OBJECTIVES AND BACKGROUND:
In head and neck cancer including hypopharyngeal carcinoma, cisplatin and 5-fluorouracil usually have been used as neoadjuvant chemotherapeutic agents. We investigated the difference in the influences of cisplatin and 5-fluorouracil (5-FU) on the p53 protein expression and cell responses (cell cycle arrest and apoptosis) in the hypopharyngeal cell line (PHUH-12).METHOD:
PNUH-12 with a mutant type p53 (one point mutation at the 78th base, C to G, in exon 7) was treated with cisplatin and 5-FU. Changes in the cell line were assessed by MTT assay, Western blotting (p53 and p21 protein), DNA fragmentation, PI stain, and DNA flow cytometry.RESULTS:
The p53 protein expression was increased after the treatment with cisplatin and 5-FU. The expression of p21 protein was increased after the treatment with 5-FU, not cisplatin. With cisplatin, we observed apoptosis by DNA fragmentation and PI stain and the increased S phase on DNA flow cytometry. But, with 5-FU, we couldn't observe apoptosis by DNA fragmentation, PI, and flow cytometry and only the increased G1 phase on DNA flow cytometry.CONCLUSION:
In hypopharyngeal cell line (PNUH-12), cisplatin induced p53 dependent apoptosis and 5-FU induced p53 and p21 dependent G0/G1 cell cycle arrest, but not apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Hypopharyngeal Neoplasms
/
Cell Line
/
Blotting, Western
/
Exons
/
G1 Phase
/
S Phase
/
Cisplatin
/
Point Mutation
/
Apoptosis
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2002
Type:
Article
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