A Mouse Model of Chronic Rhinosinusitis Induced by Streptococcus Pneumoniae / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 52-59, 2006.
Article
in Korean
| WPRIM
| ID: wpr-647544
ABSTRACT
BACKGROUND AND OBJECTIVES:
A mouse has a great potential to be used in studying genetics and inflammatory process of the rhinosinusitis. The aim of this study was to observe effects of experimentally induced chronic rhinosinusitis on histopathology of the sinonasal mucosa in a mouse and to develop a chronic form of rhinosinusitis. MATERIALS ANDMETHOD:
Thirty five, six-week old male C57BL/6 mice were used as follows 7 normal controls without intervention, 7 Sham operated controls, 7 animals with ostial obstruction alone using Merocel, 7 animals implanted with Merocel plus 106 colony forming unit (CFU)/mL of Streptococcus pneumoniae and 7 animals implanted with Merocel plus 10(8) CFU/mL of S. pneumoniae. Six weeks after intervention, the animals were sacrificed and serially sectioned at 1 mm intervals and stained with Hematoxylin and Eosin.RESULTS:
Increased epithelial thickness, goblet cell hyperplasia, epithelial disarray and inflammatory infiltration were observed in the experimental sinuses packed with Merocel alone or Merocel with bacterial inoculation, especially at the nasal septal area. However, there were no significant differences between the Merocel only inserted group and Merocel and bacteria inoculated group.CONCLUSION:
Maxillary sinus ostial obstruction or ostial obstruction with S. pneumoniae inoculation induced chronic rhinosinusitis in C57BL/6 mice as indicated by the histologic change. This study could be used as a model of chronic rhinosinusitis for further study.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Sinusitis
/
Stem Cells
/
Streptococcus
/
Streptococcus pneumoniae
/
Bacteria
/
Eosine Yellowish-(YS)
/
Goblet Cells
/
Septum of Brain
/
Genetics
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2006
Type:
Article
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