Effects of Dietary Conjugated Linoleic Acid (CLA)on Antioxidant System in the Liver of Chronically Ethanol -Treated Rats
The Korean Journal of Nutrition
;
: 105-110, 2007.
Article
in Korean
| WPRIM
| ID: wpr-648127
ABSTRACT
The study was designed to observe antioxidant activities of conjugated linoleic acid (CLA )by determining antioxidant enzyme protein levels [cytochrome P4502 E1 (CYP2E1 ), Copper, Zinc-superoxide dismutase (CuZn-SOD ), glutathione peroxidase (GSH-Px ), glutathione S-transferase (GST )] by Western blot analysis and the levels of alpha -tocopherol and 2-thiobarbituric acid reactive substances (TBARS )in the liver of chronically ethanol-treated rats. Sixty Sprague Dawley male rats were divided into 3 groups (Control, EtOH, EtOH + CLA ). All rats were fed Lieber-DeCarli liquid diet for 4 weeks by pair-feeding against the EtOH group. The liquid diet was supplemented with 1.77g CLA mixture per kg diet in the EtOH + CLA group. Isocaloric maltose dextrin was added in replace of 50g ethanol (36%kcal )for the Control group. Ethanol ingestion significantly increased the levels of CYP2E1 protein and TBARS, but significantly reduced CuZn-SOD protein level and increased GST protein level. There was no significant effect on the level of GSH-Px protein and alpha-tocopherol in the liver by ethanol. CLA supplementation with ethanol significantly increased the levels of CuZn-SOD, GSH-Px and GST and also significantly attenuated TBARS level, whereas there was no signi-ficant effect on the levels of CYP2E1 protein and alpha-tocopherol by CLA. Overall, the CLA supplemented to ethanol could significantly increase the levels of CuZn-SOD, GSH-Px and GST proteins and reduce the level of TBARS in the liver of chronically ethanol-treated rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blotting, Western
/
Thiobarbituric Acid Reactive Substances
/
Cytochrome P-450 CYP2E1
/
Copper
/
Linoleic Acid
/
Alpha-Tocopherol
/
Diet
/
Ethanol
/
Eating
/
Glutathione Peroxidase
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
The Korean Journal of Nutrition
Year:
2007
Type:
Article
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