Effects of Nasal Instillation of Staphylococcal Enterotoxin B on Allergic Inflammation in Mice / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 885-892, 2009.
Article
in Korean
| WPRIM
| ID: wpr-648398
ABSTRACT
BACKGROUND AND OBJECTIVES:
The role of Staphylococcus aureus enterotoxin B (SEB) in allergic rhinitis remains controversial. We used a mouse model of airway allergy to determine the effects of nasal instillation of SEB on allergic inflammation. MATERIALS ANDMETHOD:
BALB/c mice were intranasally sensitized with compound of Aspergillus melleus proteinase (AMp) and ovalbumin (ova) in the presence (group 3) or absence of SEB (group 2). Control mice were intranasally sensitized with either phosphate buffered saline (PBS)(group 1) or SEB (group 4) alone. The production of serum antigen-specific antibodies and total IgE, and concentrations of cytokines including interleukin-4 (IL-4), IL-5, interferon-gamma (IFN-gamma) in nasal lavage fluid (NLF) and broncoalveolar lavage fluid (BALF) were compared among the groups. Pathologic studies for eosinophilic infiltrations in the nasal mucosa and peribronchial area were also conducted.RESULTS:
Serum ova-specific immunoglobulin E (IgE) & immunoglobulin G1 (IgG1) were significantly increased in group 2 and 3 compared to group 1. The concentrations of IL-4 in BALF were significantly increased in group 2, 3, and 4 compared to group 1. The concentrations of IL-5 in BALF and NLF were significantly increased in Group 2, 3, and 4 compared to group 1. The concentrations of IFN-gamma were significantly increased in group 3 and 4 compared to group 2 although there was no significant difference among the groups in NLF. Group 2 demonstrates increased infiltration of eosinophils in the nasal mucosa and peribronchial area compared to group 3 and 4.CONCLUSION:
These results suggest that the nasal exposure to SEB did not aggravate inflammation in allergic mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aspergillus
/
Staphylococcus
/
Staphylococcus aureus
/
Immunoglobulin E
/
Immunoglobulins
/
Ovalbumin
/
Rhinitis
/
Rhinitis, Allergic, Perennial
/
Cytokines
/
Interleukin-4
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS