Localization of Cyclooxygenase Isozymes in Skin Wound Healing in Mouse / 대한해부학회지
Korean Journal of Anatomy
;
: 115-122, 2003.
Article
in English
| WPRIM
| ID: wpr-648459
ABSTRACT
Cyclooxygenase (COX)-1 and -2 expressions in the incisional wound healing of mouse skin were determined by immunohistochemistry and Western blot analysis. By Western blotting, compared to normal skin, COX-2 activity was increased at days 1, 4, 8, and 12 and was maximal at 4 day after incisional wound of mouse skin whereas COX-1 was barely detectable. In normal skin, COX-1 immunostaining was observed among the basal cells of epidermis whereas COX-2 immunostaining was detected in the more differentiated, suprabasal keratinocytes. At 1~4 days after wound, COX-2 staining was particularly prominent in the inflammatory cells, and at day 8, many macrophage-like cells were stained positively. COX-2 immunoreactive fibroblast, macrophage-like cells, and newly formed vascular endothelial cells were increased in number at 12 days after incision. These data suggest that COX-2 is constitutively expressed, just as is COX-1, in epidermis and is associated with keratinocyte differentiation. In addition, these findings support the well-established role for COX-2, the prostaglandins that they generate, as mediators of inflammatory response.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Wound Healing
/
Wounds and Injuries
/
Immunohistochemistry
/
Keratinocytes
/
Prostaglandins
/
Blotting, Western
/
Prostaglandin-Endoperoxide Synthases
/
Endothelial Cells
/
Epidermis
Limits:
Animals
Language:
English
Journal:
Korean Journal of Anatomy
Year:
2003
Type:
Article
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