Regulation of Cytokine and Cyclooxygenase Expression in Palatine Tonsils from Recurrent Tonsillitis in Children / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 765-772, 2004.
Article
in Korean
| WPRIM
| ID: wpr-648576
ABSTRACT
BACKGROUND AND OBJECTIVES:
Prostanoids are converted from arachidonic acid via cyclooxygenase (COX), which has two isoforms, COX-1 and COX-2. It has recently been shown that prostanoids may have a novel role in mediating cytokine release in some cells. We investigated the baseline expression and regulation of interleukin (IL)-6, IL-8, COX-1 and COX-2 in palatine tonsils from pediatric recurrent tonsillitis. MATERIALS ANDMETHOD:
Palatine tonsils were obtained from 12 children with recurrent tonsillitis during elective tonsillectomy and adenoidectomy. Tonsil cells were incubated with culture media only or with proinflammatory cytokines (IL-1beta and TNF-alpha) or inhibitors of transcription (actinomycin D) or translation (cycloheximide). In these groups, IL-6, IL-8, COX-1, COX-2 mRNAs and COX-1, COX-2 proteins were analyzed by reverse transcriptasepolymerase chain reaction and Western blot, respectively.RESULTS:
The baseline expressions of IL-6, IL-8, COX-1 and COX-2 mRNAs were detected although tonsillectomy was done in the silent phase without acute infection. The culture of tonsil cells with pro-inflammatory cytokines increased the expression of IL-6, IL-8 and COX-2 mRNAs and COX-2 protein. The expressions of IL-6, IL-8 and COX-2 mRNAs and COX-2 protein induced by pro-inflammatory cytokines was significantly inhibited by actinomycin D, not by cycloheximide.CONCLUSION:
Tonsil cells isolated from pediatric recurrent tonsillitis are in constantly inflamed state. The expression of COX-2 mRNA induced by pro-inflammatory cytokines is accompanied by the induction of IL- 6 and IL-8 mRNAs. The regulation of these effects occurs at the transcriptional level. Furthermore, the regulation of cytokines and COX-2 may provide an understanding of mechanism of prostanoids synthesis in chronic tonsillitis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Palatine Tonsil
/
Tonsillectomy
/
RNA, Messenger
/
Adenoidectomy
/
Tonsillitis
/
Prostaglandins
/
Blotting, Western
/
Cytokines
/
Interleukin-8
/
Interleukins
Limits:
Child
/
Humans
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2004
Type:
Article
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