Rhinovirus Infection Study Model Using Organ Culture of Turbinate Mucosa / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 632-638, 2004.
Article
in Korean
| WPRIM
| ID: wpr-648870
ABSTRACT
BACKGROUND AND OBJECTIVES:
To have better understanding on the pathophysiology of rhinovirus infection, an availability of an ideal experimental model is of utmost importance. We aimed to develop a new study model using the organ culture of turbinate mucosa to overcome the limitations of the conventional study methods. MATERIALS ANDMETHOD:
The inferior turbinate mucosae harvested during the septoturbinoplasty were cultured in air-liquid interface methods, placed on the support of gelfoam soaked in the culture media. Human rhinovirus -16 was applied on the top of the mucosal surface. The success of rhinovirus infection was determined by semi-nested RT-PCR of the mucosal surface fluid taken 48 hours after incubation. Intracellular rhinovirus was visualized by in situ hybridization. Elaboration of cytokine IL-6 and IL-8 into the culture media was quantitated using the ELISA method.RESULTS:
PCR product of 292 bp on semi-nested RT-PCR, representing successful rhinovirus infection, was detected in 5 tissues out of 10 mucosal tissues. In the in situ hybridization method, positively stained cells were found in epithelial layer in scattered fashion. In the analysis of cytokine production by continuous exposure to rhinovirus according to the time course, IL-6 and IL-8 secretions in the infected mucosae were significantly greater than in the control mucosa. The increase in the cytokine production was evident from 24 hours after the infection.CONCLUSION:
The results of this study indicate that the organ culture of turbinate mucosa could serve as an acceptable in vitro model for studying pathophysiology of rhinovirus infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Culture Techniques
/
Rhinovirus
/
Turbinates
/
Enzyme-Linked Immunosorbent Assay
/
Polymerase Chain Reaction
/
Interleukin-8
/
Interleukin-6
/
In Situ Hybridization
/
Culture Media
/
Gelatin Sponge, Absorbable
Type of study:
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2004
Type:
Article
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