The Suppressive Effect of Alendronate Sodium on Nitric Oxide Release by Macrophage / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 222-227, 2004.
Article
in Korean
| WPRIM
| ID: wpr-649061
ABSTRACT
PURPOSE:
This study was designed to present the effect of alendronate sodium on prevention of osteolysis after arthroplasty by suppression of macrophage. MATERIALS ANDMETHODS:
Submicron-sized titanium particles were made from TiAlV cylinder and plate after milling, and by repeated washing, centrifugation and floating method. 12 dogs were given alendronate by oral administration for 3 months and blood was sampled at 3 weeks, 6 weeks and 3 months. Monocytes were separated from canine blood by isopycnic centrifugation with Percoll solution and cultured in media with LPS, L-NMA, TiAlV particle (x1 SAR and x5 SAR) for 24 hours. Nitric Oxide (NO) assay by Mendelow's method and MTT assay as a cell viability test were performed.RESULTS:
Average diameter of TiAlV particles was 1.1+/-0.8 m (from 0.17 to 4.0 m). Particles less than 1.0 m were 55% and 3.0 m, 96%. Compared with control group, there was slight decrease of NO production in 3 weeks group with no statistic difference, but there was significant decrease in 6 weeks and 3 months group statistically.CONCLUSION:
Administration of alendronate for more than 6 weeks may be effective in suppression of macrophage, prevention of osteolysis after arthroplasty.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteolysis
/
Arthroplasty
/
Titanium
/
Monocytes
/
Cell Survival
/
Centrifugation
/
Centrifugation, Isopycnic
/
Administration, Oral
/
Alendronate
/
Macrophages
Limits:
Animals
Language:
Korean
Journal:
The Journal of the Korean Orthopaedic Association
Year:
2004
Type:
Article
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