Characteristic Features of Immune B Cells in Murine Cervical Lymph Node / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 241-246, 2005.
Article
in Korean
| WPRIM
| ID: wpr-649139
ABSTRACT
BACKGROUND AND OBJECTIVES:
To investigate the characteristic features of B cells in murine cervical lymph node. Animal experiment. SUBJECTS ANDMETHOD:
We obtained cervical lymph node of mice. Isolated B cells were cultured in media after the addition of several stimuli. The expression of various surface molecules to characterize lymphoid B cells were analysed by flow cytometry. Immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. B220+ cells cultured for 5 days with media alone, or LPS. Entering into S phase in response to stimuli was measured by proliferative assay.RESULTS:
Identifying phenotypic characteristics for B cells of lymph node include CD5low, CD23high, CD43low, B220high, Ig (sIg)Mlow, sIgDhigh, CD80 (B7.1)low, CD86 (B7.2)low, and Syndecam-1low. Spontaneous Immunoglobulin production did not occur in lymphoid B cells. When stimulation, IgM secretion was increased more than that of IgA and IgG. Lipopolysaccharide is a most powerful stimulus which secreted immunoglobulin. In thymidine incorporation, B cells were entered actively S phase after 48hours stimulation. CD40 plus anti-CD8 stimulated B cells to produce substantial thymidine incorporation that was much greater than those of other stimuli.CONCLUSION:
The B cells in cervical lymph node are conventional B cells (B2). Lymph node B cells secreted mainly IgM at stimuli and cell proliferation was activated at 48 hours of stimulation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Thymidine
/
Immunoglobulin A
/
Immunoglobulin G
/
Immunoglobulin M
/
Immunoglobulins
/
Enzyme-Linked Immunosorbent Assay
/
B-Lymphocytes
/
S Phase
/
Animal Experimentation
/
Cell Proliferation
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2005
Type:
Article
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