Inhibitory Effect of 15-Deoxy-Delta(12,14)-Prostaglandin J(2) on the MUC8 Expression in the Human Airway Epithelial Cells / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 435-440, 2008.
Article
in Korean
| WPRIM
| ID: wpr-649562
ABSTRACT
BACKGROUND AND OBJECTIVES:
Among the numerous mucin genes, MUC8 is regarded as one of the most important mucin genes which are related with upper airway disease such as a nasal polyp. 15-Deoxy-delta(12,14)-prostaglandin J(2)(15d-PGJ(2)), the most recently discovered prostaglandin, has been known to have multiple cellular functions, including anti-inflammatory and cytoprotective effects. However, the effect of 15d-PGJ(2) on mucin gene expression or mucin production has not yet been investigated. The purpose of this study was to determine the effect of 15d-PGJ(2) on interleukin-1beta (IL-1beta)-induced MUC8 gene expression and mucin secretion in the cultured NCI-H292 cells and human nasal polyp epithelial cells. SUBJECTS ANDMETHOD:
The MUC8 mRNA levels were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and MUC8 mucin levels were measured using an enzyme-linked immunosorbent assay (ELISA) in the cultured epithelial cells stimulated by IL-1beta. 15d-PGJ(2) was added 1 hour before stimulation.RESULTS:
15d-PGJ(2) attenuated the IL-1beta-induced MUC8 mRNA expression and mucin secretion with a dose-dependent pattern in both cultured NCI-H292 cells and human nasal polyp epithelial cells.CONCLUSION:
These results suggest that 15d-PGJ(2) may be considered as an effective agent for the control of airway mucus hypersecretion through the down-regulation of MUC8 gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Down-Regulation
/
Gene Expression
/
Nasal Polyps
/
Epithelial Cells
/
Interleukin-1beta
/
Mucins
/
Mucus
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2008
Type:
Article
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