The expression of new rat Ulip mRNA in the developing rat brains / 대한해부학회지

ABSTRACT

One cDNA was cloned out of developmentally differentially expressed genes in developing rat brains with ordered differential display method (ODD). The expression of cloned cDNA was observed from embryonal day 12 (E12), peaked at postnatal day 7 (P7), decreased to undetectable level at adult. By sequencing and sequence search with GenBank data, it was revealed that cloned cDNA was very similar to mouse Unc-33-like phosphoprotein (Ulip), which is known to be involved in the axonal outgrowth, thus, named as new rat Ulip (nrUlip). In situ hybridization histochemistry of developing rat brains showed that nrUlip mRNA was highly expressed in the area for differentiating neurons and the expression was observed just after neurogenesis in the various brain areas including thalamus, septal area, cerebral cortex, caudate-putamen, hippocampus, cerebellum, and dentate gyrus in the order of neurogenesis. These developmental expression pattern was well matched with the result of ODD. This may justify ODD as one of the best way to clone the genes differen-tially expressed among samples. These results and high sequence homology of nrUlip to mouse Ulip related with axonal outgrowth suggest that nrUlip may be also involved in the outgrowth of axon.