Dose-response Relationship of Morphologic Changes by Cisplatin and Radiation in Guinea Pig Cochleas / 대한이비인후과학회지

ABSTRACT

BACKGROUND AND OBJECTIVES: Cisplatin or radiation have been widely used in the treatment of patients with head and neck cancers. However, their adverse effects such as ototoxicity and nephrotoxicity are serious considerations. This study was aimed to establish a dose-dependent response in an animal model, with the hope of contributing to research for prevention of ototoxicity in cancer patients treated with radiation or chemotherapy. MATERIAL AND METHODS: Three different doses (4, 8, 12 mg/kg) of intraperitoneal cisplatin and radiation (17, 23, 33 Gy) were administered to guinea pigs. RESULTS: After 8 days, the temporal bones were prepared for scanning electron microscopic examination. The cochlear hair cells, especially outer hair cells in the third row, were severely damaged in the cisplatin groups and irradiation groups and these damages were related to the dose administered. The main abnormalities were the deletion or distortion of stereocilia of the hair cells and the loss of stiffness. CONCLUSION: A dose-dependent response relationship established in this study will provide as a useful reference for other researches for the prevention of ototoxicity by cisplatin and radiation.