Expresion of Collagenase-3 (Matrix Metalloproteinase-13) in human middle ear cholesteatoma and granulation / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 572-575, 2001.
Article
in Korean
| WPRIM
| ID: wpr-651386
ABSTRACT
INTRODUCTION:
Middle ear cholesteatoma is notorious for its capacity to absorb surrounding bone, but the exact mechanism of bone destruction is not fully understood until now. Recently, a group of matrix metalloproteinases (MMPs) were found to dissolve extracellular matrices. Among them, collagenase-3 (MMP-13), a newly found collagenase, is thought to be a strong enzyme to dissolve bone and cartilage. So we tried to find out whether Collagenase-3 is found in cholesteatoma and plays some role in bone destruction by cholesteatoma. MATERIALS ANDMETHOD:
We performed immunohistochemical stains on 5 cholesteatomas, 5 middle ear granulations, 5 normal middle ear mucosa and 5 deep meatal skin specimens with anticollagenase-3 antibody and analysed the staining patterns.RESULTS:
All the cholesteatomas showed strong immunoreactivity with collagenase-3. Two out of 5 granulations showed somewhat weaker and more disseminated patterns of immunoreactivity, but the rest 3 granulations showed no immunoreactivity. Normal middle ear mucosa and deep meatal skin specimens showed no immunoreactivity at all.CONCLUSIONS:
Collagenase-3 may play an active role in the process of bone destruction by cholesteatoma; however, a further study using zymography or blotting method is needed to make this clear.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Cartilage
/
Cholesteatoma
/
Collagenases
/
Cholesteatoma, Middle Ear
/
Matrix Metalloproteinases
/
Coloring Agents
/
Ear, Middle
/
Extracellular Matrix
/
Matrix Metalloproteinase 13
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2001
Type:
Article
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