Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR

Pam-Dachung LUKA; Chrisostom AYEBAZIBWE; David SHAMAKI; Frank-Norbert MWIINE; Joseph ERUME

Pam-Dachung LUKA; Chrisostom AYEBAZIBWE; David SHAMAKI; Frank-Norbert MWIINE; Joseph ERUME.

Journal of Veterinary Science ; : 323-325, 2012.

Article in English | WPRIM | ID: wpr-65159

ABSTRACT

ABSTRACT

Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.

Subject(s)

Animals; DNA Primers/analysis; Eye/virology; Goat Diseases/blood; Goats; Hair/virology; Nose/virology; Nucleoproteins/analysis; Peste-des-Petits-Ruminants/blood; Peste-des-petits-ruminants virus/genetics; Pigmentation; RNA, Viral/genetics; Reverse Transcriptase Polymerase Chain Reaction/methods; Sheep; Sheep Diseases/blood; Uganda/epidemiology

diagnosis; goats; peste des petits ruminants; sample; sheep

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Sheep Diseases / Uganda / Pigmentation / RNA, Viral / Goats / Sheep / Goat Diseases / Nose / Peste-des-petits-ruminants virus / DNA Primers Type of study: Diagnostic study Limits: Animals Country/Region as subject: Africa Language: English Journal: Journal of Veterinary Science Year: 2012 Type: Article

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