Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times
Journal of Veterinary Science
;
: 253-259, 2012.
Article
in English
| WPRIM
| ID: wpr-65168
ABSTRACT
The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70 protein expression in the heart and liver of transported pigs did not change significantly while it increased significantly (p < 0.05) in the stomach. Hsp70 mRNA levels decreased significantly (p < 0.05) in the heart after 4 h of transportation. However, mRNA expression increased significantly in the liver after 1 (p < 0.05) and 4 h (p < 0.01) of transportation, and increased significantly in the stomach of the transported pigs after 1, 4 (p < 0.01), and 2 h (p < 0.05). HSF-1 levels were reduced at 1 and 4 h (p < 0.05) only in the hearts of transported pigs. These results indicate that Hsp70 mediates distinct stress-related functions in different tissues during transportation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach
/
Stress, Physiological
/
Swine
/
Time Factors
/
Transcription Factors
/
Transportation
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Random Allocation
/
HSP70 Heat-Shock Proteins
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2012
Type:
Article
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