Visualization of Tumor Angiogenesis Using MR Imaging Contrast Agent Gd-DTPA-anti-VEGF Receptor 2 Antibody Conjugate in a Mouse Tumor Model
Korean Journal of Radiology
;
: 449-456, 2010.
Article
in English
| WPRIM
| ID: wpr-65183
ABSTRACT
OBJECTIVE:
To visualize tumor angiogenesis using the MRI contrast agent, Gd-DTPA-anti-VEGF receptor 2 antibody conjugate, with a 4.7-Tesla MRI instrument in a mouse model. MATERIALS ANDMETHODS:
We designed a tumor angiogenesis-targeting T1 contrast agent that was prepared by the bioconjugation of gadolinium diethylenetriaminepentaacetic acid (Gd-DTPA) and an anti-vascular endothelial growth factor receptor-2 (VEGFR2) antibody. The specific binding of the agent complex to cells that express VEGFR2 was examined in cultured murine endothelial cells (MS-1 cells) with a 4.7-Tesla magnetic resonance imaging scanner. Angiogenesis-specific T1 enhancement was imaged with the Gd-DTPA-anti-VEGFR2 antibody conjugate using a CT-26 adenocarcinoma tumor model in eight mice. As a control, the use of the Gd-DTPA-anti-rat immunoglobulin G (Gd-DTPA-anti-rat IgG) was imaged with a tumor model in eight mice. Statistical significance was assessed using the Mann-Whitney test. Tumor tissue was examined by immunohistochemical analysis.RESULTS:
The Gd-DTPA-anti-VEGFR2 antibody conjugate showed predominant binding to cultured endothelial cells that expressed a high level of VEGFR2. Signal enhancement was approximately three-fold for in vivo T1-weighted MR imaging with the use of the Gd-DTPA-anti-VEGFR2 antibody conjugate as compared with the Gd-DTPA-rat IgG in the mouse tumor model (p < 0.05). VEGFR2 expression in CT-26 tumor vessels was demonstrated using immunohistochemical staining.CONCLUSION:
MR imaging using the Gd-DTPA-anti-VEGFR2 antibody conjugate as a contrast agent is useful in visualizing noninvasively tumor angiogenesis in a murine tumor model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Magnetic Resonance Imaging
/
Tumor Cells, Cultured
/
Adenocarcinoma
/
Immunoenzyme Techniques
/
Colonic Neoplasms
/
Statistics, Nonparametric
/
Contrast Media
/
Gadolinium DTPA
/
Vascular Endothelial Growth Factor Receptor-2
/
Mice, Nude
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Korean Journal of Radiology
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS