Neuroprotective effects of resveratrol on 6-hydroxydopamine-induced damage of SH-SY5Y cell line / 대한수의학회지
Korean Journal of Veterinary Research
;
: 1-6, 2014.
Article
in Korean
| WPRIM
| ID: wpr-65258
ABSTRACT
Parkinson's disease is known to exhibit progressive degeneration of the dopaminergic neurons in the substantia nigra via inhibition of glutathione metabolism. It is well known that 6-Hydroxydopamine (6-OHDA) induces Parkinson's disease-like symptoms, while resveratrol (3,5,4'-trihydroxystilbene) has been shown to have anti-inflammatory and antioxidant effects. In the present study, we investigated the neuroprotective effects of resveratrol, a phytoalexin found in grapes and various plants, on 6-OHDA-induced cell damage to the SH-SY5Y human neuroblastoma cell line. Resveratrol (5 and 10 microM) inhibited 6-OHDA (60 microM)-induced cytotoxicity in SH-SY5Y cells and induced a reduction of the number of apoptotic nuclei caused by 6-OHDA treatment. Additionally, the total apoptotic rate of cells treated with both resveratrol (10 microM) and 6-OHDA (60 microM) was less than that of 6-OHDA treated cells. Resveratrol also dose-dependently (1, 5 and 10 microM) scavenged reactive oxygen species (ROS) induced by 6-OHDA in SH-SY5Y cells and prevented depletion of glutathione in response to the 6-OHDA-induced cytotoxicity in the glutathione assay. Overall, these results indicate that resveratrol exerts a neuroprotective effect against 6-OHDA-induced cytotoxicity of SH-SY5Y cells by scavenging ROS and preserving glutathione.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parkinson Disease
/
Substantia Nigra
/
Cell Line
/
Oxidopamine
/
Reactive Oxygen Species
/
Apoptosis
/
Neuroprotective Agents
/
Vitis
/
Dopaminergic Neurons
/
Glutathione
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Veterinary Research
Year:
2014
Type:
Article
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