Osteogenic Differentiation of Human Mesenchymal Stem Cells: Effects of the compositions of biodegradable polymers and the initial cell-seeding density / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 259-266, 2003.
Article
in Korean
| WPRIM
| ID: wpr-652941
ABSTRACT
PURPOSE:
To investigate the effects of initial cell-seeding density and the compositions of biodegradable polymer scaffolds on the osteogenic differentiation of human mesenchymal stem cells. MATERIALS ANDMETHODS:
Human mesenchymal stem cells (hBMSCs) were cultured three-dimensionally onto biodegradable polymers with three different compositions (65/35 PLGA, 85/15 PLGA, PLLA), and osteogenic differentiation was studied by; histology, immuno-histochemistry, and transmision/scanning electron microscopy.RESULTS:
In terms of the amount of extracellular matrix (ECM) produced, the 65/35 PLGA (23% of the whole volume) was found to produce more ECM than 85/15 PLGA (21%) and PLLA (13%). Osteoblastic differentiation of hBMSCs was induced in all scaffolds examined and the deposition of ECM with calcifications started after 2 weeks of culture. The amount of calcium deposited on the 65/35 PLGA dramatically increased at 4 weeks. On comparing low cell-seeding density with the high cell-seeding density on 65/35 PLGA, calcium deposits reached similar levels at 6 weeks.CONCLUSION:
Three-dimensional culture of hBMSCs on biodegrable polymer scaffolds induced bone formation, which was dependent on the compositions of the scaffolds and the initial cell-seeding density.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Osteogenesis
/
Polymers
/
Microscopy, Electron
/
Calcium
/
Extracellular Matrix
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Orthopaedic Association
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS