Effects of 5-Hydroxytryptamine on Potassium Currents of the Medial Vestibular Nuclear Neuron in the Rat / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 915-921, 2003.
Article
in Korean
| WPRIM
| ID: wpr-653125
ABSTRACT
BACKGROUND AND OBJECTIVES:
This study was aimed to explore the effects of the 5-hydroxytryptamine (5-HT) on potassium currents in rat vestibular nuclear neurons. MATERIALS ANDMETHOD:
Sprague-Dawley rats aged 14 to 16 days were anesthetized with ether and decapitated. After enzymatic digestion, the portion of medial vestibular nucleus neuron was removed by micropunching and gently agitated. The dissociated neurons were transferred into a recording chamber mounted on an inverted microscope and whole-cell membrane currents were recorded at room temperature by using standard patch-clamp techniques.RESULTS:
When cells were held at -70 mV and depolarized from -60 mV to +40 mV in 10 mV increments, sustained outward potassium currents were evoked. The response of medial vestibular nuclear neurons to 5-HT was not uniform. The outward potassium currents were increased in 17 of 40 cells and decreased in 23 of 40 cells. 5-carboxamidotryptamine, 5-HT1 agonist increased the outward potassium currents of the medial vestibular nuclear cell. alpha-methyl-5-hydroxytryptamine, 5-HT2 agonist decreased the outward potassium currents of the medial vestibular nuclear cell.CONCLUSION:
These results suggest that 5-HT affects the potassium currents of the cell with different effects according to the receptor subtype on which it acts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Potassium
/
Vestibular Nuclei
/
Potassium Channels
/
Serotonin
/
Rats, Sprague-Dawley
/
Patch-Clamp Techniques
/
Digestion
/
Dihydroergotamine
/
Ether
/
Serotonin 5-HT1 Receptor Agonists
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS