Expression of Vascular Endothelial Growth Factor mRNA in Endotoxin Induced Otitis Media with Effusion of the Rat / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 559-564, 1999.
Article
in Korean
| WPRIM
| ID: wpr-653205
ABSTRACT
BACKGROUND AND OBJECTIVES:
The vascular endothelial growth factor (VEGF), a heparin binding growth factor specific for endothelial cells, is both a potent enhancer of vascular permeability and an angiogenic growth factor. The purpose of this study was to investigate the semi-quantitative expression levels of multiple VEGF mRNA splicing variants in endotoxin-induced otitis media with effusion (OME) of rat. MATERIALS ANDMETHODS:
We instilled endotoxin and saline as a control into the middle ear cavity of the rat, and middle ear mucosa were taken at 0 h, 1 h, 3 h, 6 h, 12 h, 1 d, 3 d, 7 d, and 14 d. The levels of splicing variants of VEGF transcripts were evaluated by semi-quantitative RT-PCR.RESULTS:
Expression of VEGF164 mRNA and VEGF120 mRNA was first identified at 1 h after endotoxin instillation and was dramatically increased between 6 h and day 1 and then progressively decreased by day 7. Expression level of VEGF120 mRNA was 1.34+/-0.27 fold higher than that of VEGF164, and the expression level of VEGF164 was 3.89+/-0.97 fold higher than that of VEGF188.CONCLUSION:
These results suggest that VEGF may be primarily responsible for the increased vascular permeability in OME and that it seems to play a significant role in the pathogenesis of OME.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Otitis
/
Otitis Media
/
Otitis Media with Effusion
/
RNA, Messenger
/
Capillary Permeability
/
Heparin
/
Polymerase Chain Reaction
/
Endothelial Cells
/
Vascular Endothelial Growth Factor A
/
Ear, Middle
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
1999
Type:
Article
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