The Effect of Hyaluronic Acid on the Expression of MMP-13 in Healing Process of Bone Defect in Rats / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 1021-1027, 2006.
Article
in Korean
| WPRIM
| ID: wpr-653218
ABSTRACT
PURPOSE:
Matrix metalloproteinase (MMP)-13 has a degradation effect on type II collagen and is expressed in osteoblastic cells and hypertrophic chondrocytes. However, the role of MMP-13 is not completely understood. This study investigated the effect of exogenous Hyaluronic acid (HA) on the expression of MMP-13 in the healing process of bone defects. MATERIALS ANDMETHODS:
After making a bone defect in the metaphysis of the distal femur in rats, HA was instilled into the defect in the experimental group. In the control group, a bone defect was made but HA was not instilled. Two weeks after the experiment, the rats were sacrificed and the callus that filled the bone defects was obtained. A histological examination and real time reverse transcription polymeric chain reaction (RT-PCR) were performed to investigate the healing response and expression of MMP-13.RESULTS:
Histologically, the proliferation of osteoblasts and the formation of osteoid were more active in the experimental group. Nevertheless, the level of MMP-13 expression was significantly lower in the experimental group than in the control group.CONCLUSION:
From the results of this study, it appears that exogenous HA inhibits the expression of MMP-13 at the early stage of bone healing.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Polymers
/
Bony Callus
/
Chondrocytes
/
Collagen Type II
/
Reverse Transcription
/
Femur
/
Hyaluronic Acid
Limits:
Animals
Language:
Korean
Journal:
The Journal of the Korean Orthopaedic Association
Year:
2006
Type:
Article
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