Induction of Non-Steroidal Anti-Inflammatory Drug-Activated Gene(NAG-1) in Human Oral Cavity Cancer Cells and its Effect on Apoptosis / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 1030-1037, 2004.
Article
in Korean
| WPRIM
| ID: wpr-653342
ABSTRACT
BACKGROUND AND OBJECTIVES:
Nonsteroidal anti-inflammatory drug (NSAID)-activated gene (NAG-1), which is induced by NSAIDs, has proapoptotic and antitumorigenic effects in colorectal cancer cells. However, NAG-1 induction and its effect on the apoptosis in human oral cavity cancer cells (SCC 1483) have not been determined. MATERIALS ANDMETHOD:
NAG-1 expression by various NSAIDs in SCC 1483 cells was investigated by Western blot analysis. The induction of apoptosis by NSAID and the relationship between NAG-1 expression and apoptosis were determined by Western blot assay and flow cytometry. Drosophila cells stably expressing NAG-1 were constructed and NAG-1 conditioned medium (NCM) were made. Apoptosis was examined with flow cytometry on SCC 1483 cells treated with NCM.RESULTS:
Diclofenac was the most potent inducer of NAG-1. Diclofenac inhibited the proliferation of SCC 1483 cells and this inhibition was proved as apoptosis. Diclofenac induced the expression of NAG-1 and also induced apoptosis in time and dose dependent manner. In the concentrated NCM, the expression of NAG-1 was intense and apoptosis was induced by addition of 5 mu of NCM.CONCLUSION:
Based on these data, we could assure that NSAIDs induced NAG-1 in oral cavity cancer cells and NAG-1 induced apoptosis. Therefore, we suggest that it is possible to use NSAIDs as a chemopreventive agent in oral cavity cancer. Further studies on the mechanism of NAG-1 and clinical use will be needed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mouth Neoplasms
/
Colorectal Neoplasms
/
Anti-Inflammatory Agents, Non-Steroidal
/
Diclofenac
/
Blotting, Western
/
Culture Media, Conditioned
/
Apoptosis
/
Drosophila
/
Flow Cytometry
/
Mouth
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2004
Type:
Article
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