Effects of Anti-Inflammatory Drugs on the Interleukin-1 beta-Induced Cyclooxygenase-2 Expression in Human Airway Epithelial Cells / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 988-993, 2004.
Article
in Korean
| WPRIM
| ID: wpr-653355
ABSTRACT
BACKGROUND AND OBJECTIVES:
Cyclooxygenase-2 (COX-2) is a key molecule in the biosynthesis of prostaglandins, which are important inflammatory mediators in human airway inflammatory diseases. This study was designed to investigate the effects of several COX inhibitors on the interleukin-1beta (IL-1beta)-mediated COX-2 expression in human airway epithelial cells. MATERIALS ANDMETHOD:
We observed the effects of anti-inflammatory drugs such as budesonide, triamcinolone, dexamethasone, NS-398, indomethacin, salicylate and resveratrol on the IL-1beta-induced COX-2 expression in cultured human airway NCI-H292 epithelial cells. The levels of COX-2 mRNA and COX-2 protein were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.RESULTS:
NS398, reveratrol and three corticosteroids strongly suppressed the IL-1beta-mediated COX-2 expression. However, indomethacin and salicylate did not inhibit or inhibited only weakly.CONCLUSION:
The extent of IL-1beta-induced supression of COX-2 expression in the cultured human airway NCI-H292 epithelial cells depended on the kinds of anti-inflammatory drugs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Dexamethasone
/
Triamcinolone
/
Prostaglandins
/
Indomethacin
/
Blotting, Western
/
Interleukin-1
/
Prostaglandin-Endoperoxide Synthases
/
Adrenal Cortex Hormones
/
Budesonide
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2004
Type:
Article
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