Cyclooxygenase-2 Expression in Bile Duct Cancer / 한국간담췌외과학회지
Korean Journal of Hepato-Biliary-Pancreatic Surgery
;
: 172-179, 2004.
Article
in Korean
| WPRIM
| ID: wpr-65352
ABSTRACT
PURPOSE:
Epidemiological studies have shown that regular ingestion of a nonsteroidal anti-inflammatory drug reduces the risk of fatality from colon cancer. Cyclooxygenase-2 (COX-2) has been found to be overexpressed in various types of tumor, including colorectal cancer. It has been suggested that the COX-2 enzyme may play an important role in carcinogenesis and tumor progression. The aims of this study were to find the relationship between COX-2 expression and bile duct carcinogenesis and its clinical significance in bile duct cancer.METHODS:
The COX-2 expression was determined using three methods, reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemical staining. mRNA and proteins were extracted from the tissue specimens of 22 bile duct cancer patients.RESULTS:
RT-PCR and western blotting found COX-2 expressions in all the cancers and their paired noncancerous tissues. Immunohistochemistry revealed the highest levels of COX-2 expressed in bile duct carcinoma cells, mainly in the cytoplasm, and a weak reactivity of the COX-2 protein was observed in noncancerous bile duct epithelial cells.CONCLUSION:
Based on this study, it is postulated that the carcinogenesis mechanism of COX-2 expression may be initiated from a noncancerous condition, such as chronic inflammation, and may advance to bile duct cancer progression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bile
/
Bile Duct Neoplasms
/
Bile Ducts
/
RNA, Messenger
/
Immunohistochemistry
/
Colorectal Neoplasms
/
Blotting, Western
/
Polymerase Chain Reaction
/
Prostaglandin-Endoperoxide Synthases
/
Colonic Neoplasms
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Hepato-Biliary-Pancreatic Surgery
Year:
2004
Type:
Article
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