Histochemical Study on the Expression of Heat Shock Protein 70 mRNA in Developing Mouse Brain / 대한해부학회지

ABSTRACT

Using in situ hybridization technique with digoxigenin-labelled riboprobe, study on the expression of hsp 70 mRNA in the developing mouse brain was performed. The results obtained are as follows; 1. In embryonic day 16 group, cells with strong reactivity to hsp70 mRNA were found in spinal cord. In neuroepithelial layer lining fourth ventricle and external granular layer of cerebellum, moderate reactivity was observed. But the reactivity was weak in the forebrain including cerebral cortex, diencephalon and olfactory bulb. 2. In embryonic day 18 group, the regional pattern of hsp70 mRNA expression was similar to that of embryonic day 16 group. In medulla oblongata, however, stronger reactivity was found in the embryonic day 18 group. 3. In postnatal day 0 mice group, cells with moderate or strong reactivity to hsp70 mRNA were found in the overall area of central nervous system, Especially, cells with moderate reactivity were found in the dentate gyrus of hippocampus, and the supragranular cortical plate and subplate neocortex. 4. In postnatal day 2 mice group, cells with moderate or strong reactivity to hsp70 mRNA were found in the same pattern as in postnatal day 0 group. Further differentiation of cerebral cortex and cerebellum was found. 5. Strong expression of hsp70 mRNA was found in the areas with high rate of cell division. In general, the area of expression moved to more rostral area in central nervous system as development proceeds. Above results suggest that hsp70 play an important role in the development and differentiation of central nervous system.