SP1 Mediates IFNgamma-induced TIMP-1 Transctription in C6 Astroglioma Cells / 대한해부학회지
Korean Journal of Anatomy
;
: 77-83, 2007.
Article
in Korean
| WPRIM
| ID: wpr-653610
ABSTRACT
Tissue inhibitors of metalloproteinases (TIMPs) comprise a family of secreted multifunctional proteins that consists of four members (TIMP-1 to TIMP-4). TIMPs are all major inhibitors of most matrix metalloproteinases (MMPs). They are synthesized by a variety of different cells and regulated by a number of cytokines and by growth and differentiation factors. The balance between MMPs and TIMPs plays a crucial role in the turnover of extracellular matrix in normal and pathological conditions. Here, we report that the production of TIMP-1 was upregulated in interferon (IFNgamma-treated C6 astroglioma cells and that the proximal 226 bp region of the promoter of the TIMP-1 gene is responsible for IFNgamma-induced induction in C6 astroglioma cells. The induction of TIMP-1 production by IFNgamma was virtually abolished by introducing mutations into the putative SP1-response element in the promoter, indicating that the SP1 binding site conferred responsiveness onto a heterologous promoter. Together the results suggest that the IFNgamma-induced upregulation of TIMP-1 production in C6 astroglioma cells is mediated by the SP1 binding site localized in the TIMP-1 gene promoter.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Astrocytoma
/
Binding Sites
/
Up-Regulation
/
Cytokines
/
Interferons
/
Tissue Inhibitor of Metalloproteinase-1
/
Matrix Metalloproteinases
/
Metalloproteases
/
Extracellular Matrix
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Anatomy
Year:
2007
Type:
Article
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