Effects of Chongkukjang Intake on Lipid Metabolism and Liver Function in Ethanol Consumed Rats

ABSTRACT

This study was performed to investigate the effect of chongkukjang intake on lipid metabolism and liver function in ethanol consumed rats. Twenty one Sprague-Dawley male rats aging 4 weeks old were used as experimental animals, which were divided into three dietary groups casein diet (CA), soybean diet (SB) and chongkukjang diet (CJ). Alcohol was consumed with water as 25% (v/v) ethanol solution. After 4 weeks of experimental period, rats were sacrificed to get blood and liver samples for analysis of lipids, lipid peroxides, antioxidative enzymes and biochemical indices of liver function. The mean body weight, food intake and liver index were not significantly different among three groups. Serum level of total lipid, triglyceride and total cholesterol of chongkukjang diet group was the lowest among three groups although the difference was not significant. HDL-cholesterol level was significantly (p < 0.05) higher in chongkukjang diet group than that of casein diet group. LDL-cholesterol level of chongkukjang and soybean diet group was significantly (p < 0.05) lower than that of casein diet group respectively. Liver TBARS of chongkukjang and soybean diet group was significantly (p < 0.05) lower than that of casein diet group respectively. The superoxide dismutase activity of chongkukjang diet group was significantly (p < 0.05) higher than that of casein diet group. Catalase activity was not significantly different among three groups. As indices of liver function, glutamic oxaloacetic transminase (GOT), glutamic pyruvic transminase (GPT), gamma-glutamyl transpeptidase (gamma-GTP) and lactate dehydrogenase (LDH) were not significantly different among three groups. Serum alcohol concentration and activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were not significantly different among three groups. The chongkukjang diet seems to give a beneficial effect for improving lipid metabolism by increasing HDLcholesterol level and SOD activity while reducing liver TBARS level. However, effect on liver function has to be investigated further.