The Ototoxicity of Cisplatin is Mediated by NO: A Study using L-NAME and MK-801 in Guinea pigs / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 741-746, 2002.
Article
in Korean
| WPRIM
| ID: wpr-654571
ABSTRACT
BACKGROUND AND OBJECTIVES:
Nitric oxide has been suggested to play an important role in the pathogenesis of cisplatin ototoxicity. L-NAME (NG-Nitroarginine Methyl Ester) is an inhibitor of nitric oxide synthase. MK-801 (Dizocilpine Maleate) is a NMDA receptor antagonist. To evaluate a role of nitric oxide in cisplatin ototoxicity, we investigated whether L-NAME and MK-801 can block the cisplatin ototoxicity in guinea pigs. MATERIALS ANDMETHOD:
In the Group 1, normal saline was injected intraperitoneally as a control group. Group 2, 3, 4, and 5 were injected intraperitoneally as described in the following Group 2, cisplatin only; Group 3, L-NAME+isplatin; Group 4, MK-801+cisplatin; Group 5, L-NAME+K-801+cisplatin. Using an auditory brainstem response, hearing threshold was tested before cisplatin administration and 5 days after cisplatin injection in each group. The morphological changes of the cochlea were observed by scanning electron microscopy.RESULTS:
In the Group 2, a significant hearing loss was observed comparing to Group 1. In contrast , Group 3, 4, and 5 did not demonstrate any significant hearing loss compared to Group 1. In the scanning electron microscopy, the Group 2 showed distorsion and loss of stereocilia of the hair cells. However, the Group 1, 3, 4, and 5 demonstrated well preserved cochlear hair cell morphology.CONCLUSION:
Hearing loss induced by ototoxicity of cisplatin was prevented by L-NAME and MK-801. This study suggests that NO may mediate cisplatin ototoxicity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Microscopy, Electron, Scanning
/
Dizocilpine Maleate
/
Evoked Potentials, Auditory, Brain Stem
/
N-Methylaspartate
/
Cisplatin
/
Guinea
/
Cochlea
/
Nitric Oxide Synthase
/
NG-Nitroarginine Methyl Ester
/
Stereocilia
Limits:
Animals
Country/Region as subject:
Africa
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2002
Type:
Article
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