Construction of a New T-Vector: Nickase (Nt.BspQI)-Generated T-Vector Bearing a Reddish-Orange Indicator Gene
Tissue Engineering and Regenerative Medicine
;
(6): 66-69, 2016.
Article
in English
| WPRIM
| ID: wpr-654675
ABSTRACT
T-vectors are widely used for cloning the polymerase chain reaction (PCR) products. However, the low conversion efficiency of a plasmid into the linear T-vector usually results in non-recombinants. Here, we designed a new plasmid pNBQ-T to easily select the recombinant colonies harboring PCR products. pNBQ-T plasmid, which contains a DsRed indicator gene between two Nt.BspQI restriction cassettes, each of which contains palindromic sequences susceptible to Nt.BspQI nickase (5′-GCTCTTCT
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Polymerase Chain Reaction
/
Clone Cells
/
Cloning, Organism
/
Deoxyribonuclease I
/
Myostatin
/
Methods
Language:
English
Journal:
Tissue Engineering and Regenerative Medicine
Year:
2016
Type:
Article
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