Neurotoxic Effect of Streptozotocin and Neuroprotective Effect of Insulin Growth Factor-II to the Cultured Mouse Schwann Cells / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 577-581, 2000.
Article
in Korean
| WPRIM
| ID: wpr-655096
ABSTRACT
BACKGROUND:
The sensorineural hearing loss due to diabetes is progressive and bilateral, and predominantly occurs in the old, although its accurate pathogenesis is still unknown.Objectives:
The purpose of this study is to clarify the neurotoxic effect of streptozotocin (STZ) and the neuroprotective effect of insulin-like growth factor-II(IGF-II) on the cultured Schwann cells of cohlear nerve. MATERIALS ANDMETHODS:
MTT assays were performed on cultured mouse Schwann cells which were treated with various concentrations of STZ for 24 hours, and the neuroprotective effect of IGF-II against STZ-induced neurotoxicity were also examined.RESULTS:
1) MTT50 value was the concentration of 40 pM STZ (highly toxic MTT50<100 pM), 2) Cell viability of cultured mouse Schwann cells treated with STZ markedly decreased in a dose-dependent manner.CONCLUSION:
It is suggested that STZ induces a severe toxic effect on cultured Schwann cells of mouse, and selective neurotrophic factors such as IGF-II are very effective in preventing the neurotoxicity induced by STZ.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Schwann Cells
/
Insulin-Like Growth Factor II
/
Cell Survival
/
Streptozocin
/
Neuroprotective Agents
/
Hearing Loss, Sensorineural
/
Insulin
/
Nerve Growth Factors
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS