c-kit and Ligand Expression in the Mouse Hippocampus after Kainate-induced Seizure / 대한해부학회지
Korean Journal of Anatomy
;
: 87-98, 1997.
Article
in Korean
| WPRIM
| ID: wpr-655722
ABSTRACT
In the present study, we have etamined the role of c-kit and KL ligand in the mouse brain after kainate-induced seizure. To investigate whether c-kit receptor and KL ligand might involved in kainate-induced apoptosis, the expression patterns of c-kit and KL mRNA and localization of immunoreactivity for c-Kit, SCF and Bcl-2 protein were examined by in situ hybridization technique and immunohistochemical method, respectively, in the mouse hippocampus after kainate treatment. This report is the first demonstration for the role of c-kit receptor and KL ligand in the kainate-induced apoptosis. Our conclusion is based on 1] c-kit and KL mRNA expressions were increased in CA3 region of the hippocampus in 1h after kainate treatment, 2] immunoreactivities for c-Kit protein and SCF were detected higher level in the CA1 and CA3 sectors in 24h after kainate treatment, 3] expression level for Bcl-2 protein was increased in the CA3 region of the hippocampus 24h after kainate treatment. These results suggest that bcl-2 could promote cell survival of injured neurons in CA3 after kainate-induced seizure. And increased translations of c-kit receptor and KL ligand after kainate injection in this area susgest that c-kit receptor and KL ligand could have a role in the kainate-induced apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Seizures
/
Translations
/
Brain
/
RNA, Messenger
/
Cell Survival
/
In Situ Hybridization
/
Apoptosis
/
Proto-Oncogene Proteins c-kit
/
Hippocampus
/
Kainic Acid
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Anatomy
Year:
1997
Type:
Article
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